cloning of luxab reporter gene into iranian plant pathogen bacterial isolates pseudomonas syringae and ralostonia solanacerum

abstract the ability to monitor particular microorganism in an environment is a difficult task. many genes permit the differentiation of strains by conferring production of unique phenotypes such as bioluminescence [marine bacterial luciferase (luxab)]. therefore, lux-ab gene was cloned into two endemic plant pathogens pseudomonas syringae and ralostonia solanacearum by electrotransformation. lux-marking of above strains was carried out using minitn-5 luxab transposon. purified strains were transformed with plasmid put containing luxab gene by electroporator. electroporation was performed in voltage 2/5 kv for 5 milisecend. all luxab marked strains could grow on kb agar medium containing 12.5 µg/ml tetracycline and their luminescence intensity was measured by luminometer. lux-marked p. syringae and r. solanacearum were stable genetically engineered strains making them quite appropriate.